Coding

Part:BBa_K4165149:Design

Designed by: Hossam Hatem   Group: iGEM22_CU_Egypt   (2022-09-30)


T7 promoter-6x His tag-HtrA1-T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 181
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was optimized to be suitable for expression in E.coli


Source

synthetic protein retrieved from literature.

References